The new medium reduced the production cost by 85% compared to the conventional de Man, Rogosa and Sharpe (MRS) medium.
This optimized medium enhanced the antimicrobial activity of postbiotic RS5 by 108% compared to standard methods, achieving a potency of rs5.rar
A study published in Scientific Reports utilized a combination of conventional and statistical-based approaches—specifically Fractional Factorial Design and Central Composite Design of Response Surface Methodology (RSM)—to refine the growth medium. The new medium reduced the production cost by
Postbiotic RS5, derived from Lactiplantibacillus plantarum RS5, has emerged as a promising alternative to antibiotics in livestock feed due to its significant antimicrobial activity. To make this functional supplement commercially viable, research has focused on optimizing the production medium to maximize output while minimizing costs. The Optimization Study derived from Lactiplantibacillus plantarum RS5