The title serves as a double entendre. While the medical team searches for a genetic cause for Dan’s illness, House becomes obsessed with a discrepancy: Dan’s parents both have blue eyes, but Dan has green eyes. The Medical Reality

We see the start of their "negotiation" style, where House uses a legal loophole (a bet over a clinic patient) to get the tests he wants.

of this episode to real-life SSPE cases.