: New leaves are preferred because mature leaves contain higher concentrations of polyphenolic compounds and polysaccharides , which make the grinding process difficult and interfere with DNA purity.
Researchers compared the efficiency of DNA extraction from new 7-day-old flushes versus . matures old 50
: Using a spectrophotometer, DNA concentration in old leaves ranged from 981.8 ng/µl to 398.34 ng/µl , showing higher variability and potential contamination compared to younger tissue. : New leaves are preferred because mature leaves