Gram Staining | Bacterial Staining Technique | Microbiology | Vivek Srinivas | #bacteriology -

First, he flooded the slide with . The bacteria soaked it up, turning a deep, royal purple. “Phase one,” he whispered. “The Primary Stain.” At this stage, every microbe on the slide looked identical, cloaked in violet armor.

The world beneath the glass exploded into color. Amidst the sea of deep (the Gram-Positive titans), there were now bright pink specks (the Gram-Negative rebels). The mystery was gone; the identity of the colony was clear.

Next came the . This was the "mordant," the chemical glue. It bound to the crystal violet inside the cell walls, creating large complexes that wouldn't easily leave.

He reached for the four sacred vials of the technique.