C4.zip

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: The protein sample is aspirated through the tip, binding to the C4 resin.

: Contaminants are washed away with a dilute acid, such as trifluoroacetic acid (TFA) .

: Typically optimized for very small volumes (microliters).

: Best for proteins > 10 kDa and highly hydrophobic peptides.

A C4 ZipTip is a standard pipette tip containing a microscopic bed of resin fixed at its distal end. Unlike C18 resins, which are used for smaller peptides, the C4 resin is optimized for larger, more hydrophobic molecules like proteins and large polypeptides. Primary Uses in Research Researchers use these tools for two main reasons:

: The purified protein is eluted directly onto a target plate using an organic solvent like acetonitrile , where it is mixed with a matrix for analysis. Key Specifications Resin Type : Butyl (C4) silica.

Expression, Purification and Characterization of the Human ... - Nature

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C4.zip

: The protein sample is aspirated through the tip, binding to the C4 resin.

: Contaminants are washed away with a dilute acid, such as trifluoroacetic acid (TFA) . c4.zip

: Typically optimized for very small volumes (microliters). : The protein sample is aspirated through the

: Best for proteins > 10 kDa and highly hydrophobic peptides. : Best for proteins > 10 kDa and highly hydrophobic peptides

A C4 ZipTip is a standard pipette tip containing a microscopic bed of resin fixed at its distal end. Unlike C18 resins, which are used for smaller peptides, the C4 resin is optimized for larger, more hydrophobic molecules like proteins and large polypeptides. Primary Uses in Research Researchers use these tools for two main reasons:

: The purified protein is eluted directly onto a target plate using an organic solvent like acetonitrile , where it is mixed with a matrix for analysis. Key Specifications Resin Type : Butyl (C4) silica.

Expression, Purification and Characterization of the Human ... - Nature

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